The Infectious disease assay development laboratory is a BSL2 facility occupying 372 sq foot space, adjacent to the KU-HTS laboratory. The laboratory space provides an appropriate environment for handling of biohazardous infectious, viable, whole organisms. The use of infectious disease organisms in a screen increases the the probability that chemical hits identified during a screen will retain efficacy and specificity when used in subsequent biologically relevant studies. In addition to a dedicated autoclave (10sq ft ) that is readily available for contained biohazard waste processing, the laboratory is equipped with freezers (-300C and -800C), a refrigerator, multi-modal BioTek Synergy Neo 2 plate reader, incubator shaker, cell growth incubator, centrifuges, and biosafety cabinets. The BSL-2 laboratory facilities enable cultivation and screening of pathogenic bacterial and fungal strains, as well as mammalian cells, development and optimization of in vitro biochemical and cell based assays, (bacterial, fungal, algal, mammalian) and also provides compound collections for assay validation and screening.
The IDAD laboratory has a collection of 40,000 compounds, obtained from the following sources:
(1) KU-CMLD Collection: The University Of Kansas Center Of Excellence in Chemical Methodologies & Library Development (KU CMLD) has synthesized libraries utilizing new principles of scaffold design. The 5160 compounds have drug-like characteristics and pharmacological activity based on established drug design principles. (NIH CCET CoBRE funding: 8P30GM103495)
(2) Analyticon Natural products library: A collection of 4196 compounds, purified (>85%) compounds isolated from plants (MEGxp) and microorganisms (MEGxm).(NIGMS Chemical Biology of Infectious Disease grant P20GM113117 03)
(3) ChemDiv Peptidomimetics library: A collection of 12,139 organic molecules that mimic the activity of peptides, a relatively unexplored area with a high potential of obtaining hits.(NIGMS Chemical Biology of Infectious Disease grant P20GM113117 03)
(4) ChemDiv 3D Biodiversity: A collection of 19,528 scaffolds identified from the 3D clustered biologically annotated chemical space.(NIGMS Chemical Biology of Infectious Disease grant P20GM113117 03).